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Biotechnology : Principles and Processes
Question
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Restriction endonucleases are enzymes which

  • make cuts at specific positions within the DNA molecule

  • recognize a specific nucleotide sequence for binding of DNA ligase

  • restrict the action of the enzyme DNA polymerase

  • remove nucleotides from the ends of the DNA

Answer

A.

make cuts at specific positions within the DNA molecule

Restriction endonucleases recognise a specific DNA base sequence (recognition sequence of the recognition site, restriction sequence or restriction site) and cleave both the strands of DNA at or near the site. The enzyme cuts are DNA, generating restriction fragments wit overhanging ends or blunt ends.

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