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Biotechnology : Principles And Processes

Question

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Define “melting of target DNA”.

Solution

The target DNA containing sequence to be amplified is heat denatured (around 94°C for 15 seconds) to separate its complementary strands. the high temperature causes the hydrogen bond to break and thus the strands are separated and this is called melting of target DNA.

Some More Questions From Biotechnology : Principles and Processes Chapter

Define T-DNA.

Name a technique by which DNA fragments can be separated.

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

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