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Biotechnology : Principles And Processes
Question
Do eukaryotic cells have restriction endonucleases ? Justify your answer.
Solution
No, eukaryotic cells do not have restriction endonucleases. This is because the DNA of eukaryotes is highly methylated by a modification enzyme, called methylase. Methylation protects the DNA from the activity of restriction enzymes .These enzymes are present in prokaryotic cells where they help prevent the invasion of DNA by virus.
Some More Questions From Biotechnology : Principles and Processes Chapter
What are the different tools in Recombinant DNA technology?
Give the full form of PCR. Who developed it ?
What is the source of DNA polymerase i.e., Taq polymerase ?
Define “melting of target DNA”.
What would be the molar concentration of human DNA in a human cell ? Consult your teacher.
Do eukaryotic cells have restriction endonucleases ? Justify your answer.
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?
Expand EFB.
What are two core techniques that enabled birth of biotechnology ?
What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.
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