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Biotechnology : Principles and Processes
Question
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The introduction of t-DNA into plates involves

  • Infection of the plant by agrobacterium tumefaciens

  • Altering the pH of the soil, then heat-shocking the plants

  • Exposing the plates to cold for a brief period

  • Allowing the plant roots to stand in water

Answer

A.

Infection of the plant by agrobacterium tumefaciens

Agrobacterium tumefaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as tDNA to transform normal plant cells into a tumour and direct these tumour cells to produce the chemicals required by the pathogen. 

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Some More Questions From Biotechnology : Principles and Processes Chapter

Define T-DNA.

Name a technique by which DNA fragments can be separated.

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

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