☰
✕

Biotechnology : Principles and Processes
Question
Wired Faculty App

The cutting of DNA at specific locations become possible with the discovery of

  • Restriction enzymes

  • Probes

  • Selectable markers

  • Ligases

Answer

A.

Restriction enzymes

Restricition enzymes are DNA cutting enzymes found in bacteria. A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides. For example, the bacterium Haemophilus aegyptius produces an enzyme named Hae III that cuts DNA wherever, it encounters the sequence.
5'-G G C-3'
3'-C C G G-5'

Sponsor Area

Some More Questions From Biotechnology : Principles and Processes Chapter

What is crown gall tumor ?

Define T-DNA.

Name a technique by which DNA fragments can be separated.

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?