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Biotechnology : Principles and Processes
Question
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Rearrange the following in the current sequences to accomplish an important biotechnological reaction :

(a) In vitro synthesis of region of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

Answer

The given step refer to the steps involved in the polymerase chain reaction:
(b) Chemically synthesized oligonucleotides
(f) Nucleotides provided
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
(g) Primers
(e) Genomic DNA template
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(a) In vitro synthesis of region of DNA of interest

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