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Biotechnology : Principles and Processes
Question
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How is  Fragmentation of DNA of interest carried out in recombinant DNA technology ?

Answer

Fragmentation DNA of interest  is  carried out by incubating the purified DNA molecules with suitable restriction enzymes at optimal conditions for the particular enzyme. The restriction endonulease cut the DNA molecules at specific recognition sites and thus fragment he DNA. 

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Some More Questions From Biotechnology : Principles and Processes Chapter

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

What are two core techniques that enabled birth of biotechnology ?

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

List three important features necessary for preparing genetically modified organism.