Biotechnology : Principles and Processes
No, eukaryotic cells do not have restriction endonucleases. This is because the DNA of eukaryotes is highly methylated by a modification enzyme, called methylase. Methylation protects the DNA from the activity of restriction enzymes .These enzymes are present in prokaryotic cells where they help prevent the invasion of DNA by virus.
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What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
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With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.
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