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Biotechnology : Principles and Processes
Question
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Select the correct order of processing of PCR.

  • Extension, primer annealing, denaturation

  • Denaturation, primer annealing, extension

  • Denaturation, extension, primer annealing

  • Primer annealing, denaturation, extension

Answer

B.

Denaturation, primer annealing, extension

Polymerase chain reaction (PCR) is a technique to amplify a selected DNA sequence by a million fold or more. In this procedure, the DNA containing the sequence to be amplified is denatured by heating. This denatured DNA is then annealed to the synthetic oligonucleotide primers. Then Taq polymerase is used to replicate the DNA segment between the sites complementary to olipgonucleotide primer and extend it.

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