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Biotechnology : Principles and Processes
Question
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How are transformants selected from nontransformants

  • Presence of more than one recognition site in the vector DNA.

  • Presence of alien DNA into the vector DNA results into insertional inactivation of selectable marker.

  • Antibiotic resistance gene gets inactivated due to insertion of alien DNA.

  • Both (b) and (c).

Answer

D.

Both (b) and (c).

Selectable markers helps in identifying and eliminating nontransformants and selectively permitting the growth of the transformants.

The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes In this case, one antibiotic resistance gene helps in selecting the transformants, whereas the other antibiotic resistance gene gets ‘inactivated due to insertion’ of alien DNA, and helps in selection of recombinants.

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Or
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