Biotechnology : Principles and Processes
Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
If both assertion and reason are true and reason is the correct explanation of assertion
If both assertion and reason are true but reason is not the correct explanation of assertion
If assertion is true but reason is false
If both assertion and reason are false
C.
If assertion is true but reason is false
A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within the molecule known as restriction sites. Once restriction enzyme finds a recognition sequence, it binds and cut each of the two strands of the double helix at specific point leaving single stranded portions at the ends. This resulta in the overhanging stretches known as sticky ends.
These are so named because they form hydrogen bonds with their complementary counter parts ,ie, they can join similar complementary ends of DNA fragment from some other source with the help of DNA ligase. This stickiness of the ends facilitates the action of the enzyme DNA ligase, not DNA polymerase.
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