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Biotechnology : Principles and Processes
Question
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

  • centrifugation

  • polymerase chain reaction

  • electrophoresis

  • restriction mapping

Answer

C.

electrophoresis

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by electrophoresis. The polymerase chain reaction is simply DNA replication in a test tube. Restriction mapping is the process of obtaining structural information on a piece of DNA by the use of restriction enzymes, e.g., endonucleases that recognise specific 4 to 8 base regions of DNA.

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