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Biotechnology : Principles And Processes

Question
CBSEENBI12002408
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Which one of the following represents a palindromic sequence in DNA?

  • 5´ - GATACC - 3´
    3´ - CCTAAG - 5

  • 5´ - GAATTC - 3
    3´ - CTTAAG - 5´

  • 5´- CCAATG - 3´
    3´ - GAATCC - 5´

  • 5´ - CATTAG - 3´
    3´ - GATAAC - 5

Solution

B.

5´ - GAATTC - 3
3´ - CTTAAG - 5´

In a palindrome, the base sequence in the second half of a DNA strand is the mirror image of sequence in first half. But in palindrome with rotational symmetry the base sequence in first half of one strand is mirror image of second half of complementary strand, e.g., 
5´ - GATACC - 3´
3´ - CCTAAG - 5´

Some More Questions From Biotechnology : Principles and Processes Chapter

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

What are two core techniques that enabled birth of biotechnology ?

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

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