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Biotechnology : Principles And Processes

Question
CBSEENBI12002385
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Biolistics (gene-gun) is suitbale for

  • Disarming pathogen vectors

  • Transformation of plant cells

  • Constructing recombinant DNA by joing whith vectors

  • DNA finger printing

Solution

B.

Transformation of plant cells

In biolistic or gene gun method 1-2 straight mug tungsten or gold particle (called microprojectile) coated with DNA to be used for transformation are accelerated to velocities which enable their entry into plant cells/nuclei. This technique has general applicability to plant species and can be used to deliver DNA into virtually all tissues.

Some More Questions From Biotechnology : Principles and Processes Chapter

What is crown gall tumor ?

Define T-DNA.

Name a technique by which DNA fragments can be separated.

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

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