The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
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Non-recombinant bacteria containing β-galactosidease
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Insertional inactivation of α - galactosidase in recombinant bacteria
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Insertional inactivation of α-galactosidase in recombinant bacteria
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Inactivation of glycosidase enzyme in recombinant bacteria
C.
Insertional inactivation of α-galactosidase in recombinant bacteria
The colonies of recombinant bacteria appear white in contrast to blue calories of non-recombinant bacteria because of insertional inactivation of alpha-galactosidase in recombinant bacteria. Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoprotein. It in encoded by theGLA gene. β-galactosidase is an exoglycosidase, which hydrolyzes the β-glycosidic bond formed between a galactose and its organic rhoiety.
