Question
The figure below is the diagrammatic representation o the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components(s)?
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Ori- original restriction enzyme
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rop-reduced osmotic pressure
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Hind III, Eco RI-selectable markers
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ampR , tetR -antibiotic resistance genes
Solution
D.
ampR , tetR -antibiotic resistance genes
Ori represents the site of origin of replication, rop represents the proteins that take part in the replication of plasmid. Hind III, Eco RI are the recognition sites of restriction endonucleases. ampR and tetR are the antibiotic resistant gene parts.