Question
The introduction of t-DNA into plates involves
-
Infection of the plant by agrobacterium tumefaciens
-
Altering the pH of the soil, then heat-shocking the plants
-
Exposing the plates to cold for a brief period
-
Allowing the plant roots to stand in water
Solution
A.
Infection of the plant by agrobacterium tumefaciens
Agrobacterium tumefaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as tDNA to transform normal plant cells into a tumour and direct these tumour cells to produce the chemicals required by the pathogen.