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Biotechnology : Principles And Processes

Question
CBSEENBI12002276

The introduction of t-DNA into plates involves

  • Infection of the plant by agrobacterium tumefaciens

  • Altering the pH of the soil, then heat-shocking the plants

  • Exposing the plates to cold for a brief period

  • Allowing the plant roots to stand in water

Solution

A.

Infection of the plant by agrobacterium tumefaciens

Agrobacterium tumefaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as tDNA to transform normal plant cells into a tumour and direct these tumour cells to produce the chemicals required by the pathogen.