Question
The cutting of DNA at specific locations become possible with the discovery of
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Restriction enzymes
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Probes
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Selectable markers
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Ligases
Solution
A.
Restriction enzymes
Restricition enzymes are DNA cutting enzymes found in bacteria. A restriction enzyme recognizes and cuts DNA only at a particular sequence of nucleotides. For example, the bacterium Haemophilus aegyptius produces an enzyme named Hae III that cuts DNA wherever, it encounters the sequence.
5'-G G C-3'
3'-C C G G-5'
