Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.

Solution

The technique to obtain multiple copies of a gene is PCR - Polymerase Chain rection.
It invoves the following steps.
Denaturing – The double-stranded template DNA is heated to separate it into two single strands.

Annealing – The temperature is lowered to enable the DNA primers to attach to the template DNA.

Extending – The temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme.

These three stages are repeated 20-40 times, doubling the number of DNA copies each time.

Some More Questions From Biotechnology : Principles and Processes Chapter

Name the soil bacterium which contain gene for production of endotoxins.

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Biotechnology : Principles And Processes

Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.

Some More Questions From Biotechnology : Principles and Processes Chapter

Name the soil bacterium which contain gene for production of endotoxins.

What is crown gall tumor ?

Define T-DNA.

Name a technique by which DNA fragments can be separated.

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

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