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Biotechnology : Principles And Processes

Question
CBSEENBI12001825
Wired Faculty App

Rearrange the following in the current sequences to accomplish an important biotechnological reaction :

(a) In vitro synthesis of region of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA

Solution
The given step refer to the steps involved in the polymerase chain reaction:
(b) Chemically synthesized oligonucleotides
(f) Nucleotides provided
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
(g) Primers
(e) Genomic DNA template
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(a) In vitro synthesis of region of DNA of interest

Some More Questions From Biotechnology : Principles and Processes Chapter

What is crown gall tumor ?

Define T-DNA.

Name a technique by which DNA fragments can be separated.

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

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