(a) Identify (A) and (B) illustrations in the following:
i.
ii.
(b) Write the term given to (A) and (C) and why?
(c) Expand PCR. Mention its importance in biotechnology.
(a) A represents - Sticky end.
B represents - Foreign DNA insert.
(b) The term used for A is Sticky ends, it is called so because they form hydrogen bonds with their complementary cut counterparts. Their stickiness of the ends facilitates the action of the enzyme DNA ligase.
C are called the palindromic nucleotide sequence. These are named so because the sequence of base pairs reads same on the two strands when orientation of reading is kept the same
(c) PCR stands for Polymerase Chain Reaction. PCR is a technique used to synthesize multiple copies of the gene (or DNA) of interest in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. It is extensively used in the process of gene manipulation.