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Biotechnology : Principles And Processes

Question

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How and why is the bacterium Thermus aquaticus employed in recombinant DNA techonolgy ? Explain.

Solution

Thermus aquaticus bacterium is used in recombinant DNA technology to provide the enzyme Taq Polymerase which is thermostable enzyme which can even work which remain active during the high temperature induced denaturation of double stranded DNA. It is used in PCR to amplify the DNA segments.

Some More Questions From Biotechnology : Principles and Processes Chapter

Define T-DNA.

Name a technique by which DNA fragments can be separated.

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

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