Question
How is DNA isolated in purified form ?
Solution
The steps to isolate DNA in purified form are -:
1.The cell is broken up to release the DNA molecules along with other macromolecules such as RNA, proteins, polysaccharides and also lipids. This is done by treating the bacterial cells/plant or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), chitinase (fungus).
2. The RNA is removed by treatment with ribonuclease.
3. The proteins are removed by treatment with protease.
4.Ethanol is added to precipitate the DNA.
5. The purified DNA can be collected in the form of fine threads in the suspension.
