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Biotechnology : Principles And Processes

Question
CBSEENBI12000909
Wired Faculty App

Discuss with your teacher and find out how to distinguish between

(a) Plasmid DNA and chromosomal DNA
(b)Exonuclease and endonuclease.

Solution
(a) Differences between plasmid DNA and chromosomal DNA

Plasmid DNA

Chromosomal DNA

1. It is self replicating, DNA molecule found naturally in many bacteria and yeast.

2. It is not essential for normal growth and division and contains information for a few traits.

1. Chromosomal DNA present in chromosomes of all organisms.

2. It is essential for growth and division and contains information for all traits.

(b) Difference between exonuclease and endonuclease

Exonuclease

This enzyme removes nucleotides from the terminals ,either from 5' or 3' ends of DNA molecules.

Endonuclease

It cuts the DNA at specific position of nitrogen bases within  the length of DNA except the ends.

 

Some More Questions From Biotechnology : Principles and Processes Chapter

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

What are two core techniques that enabled birth of biotechnology ?

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

List three important features necessary for preparing genetically modified organism.

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