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Biotechnology : Principles And Processes

Question
CBSEENBI12000899

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

Solution

Action of restriction enzyme

EcoRI cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA.

Steps in formation of recombinant DNA by action of restriction endonuclease enzyme - EcoRI