How is recombinant DNA transferred to host ?

Solution

Transfer of recombinant DNA into the host is done by the following methods :

1. The bacterial cells are made competent to take up recombinant DNA by treating them with a specific concentration of calcium, that increases the efficiency with which DNA enters the cell through the pores in its cell wall.

2. Microinjection is a method in which the recombinant DNA is directly injected into the nucleus-of the animal cell with the help of mirco-needles or micropipettes.

3. Gene gun or biolistics is a method suitable for plant cells, where plant cells are bombarded with high-velocity microparticles of gold or tungsten coated with DNA.

4. Disarmed pathogens can be allowed to infect the host cell, which then transfer the recombinant DNA into the host.

5. Electroporation in which electricity is applied to create transient pores in the host cell so that it takes up Recombinant DNA with ease.

Some More Questions From Biotechnology : Principles and Processes Chapter

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

What are two core techniques that enabled birth of biotechnology ?

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

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Biotechnology : Principles And Processes

How is recombinant DNA transferred to host ?

Some More Questions From Biotechnology : Principles and Processes Chapter

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

What are two core techniques that enabled birth of biotechnology ?

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

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For Daily Free Study Material Join wiredfaculty

Biotechnology : Principles And Processes

How is recombinant DNA transferred to host ?

Some More Questions From Biotechnology : Principles and Processes Chapter

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

What are two core techniques that enabled birth of biotechnology ?

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.OrWith the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

Mock Test Series

NCERT Sample Papers

Entrance Exams Preparation

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.