Question
What is PCR? List the three main steps. Show the steps with a diagrammatic sketch.
Solution
PCR is Polymerase Chain Reaction. It is used to amplify the DNA that is make multiple copies of the desired DNA 'in vitro' using DNA polymerase and two sets of primers.
Three main steps of PCR are-
1. Denaturation- where the DNA strands are heated so that the two strands separate and form single strands to which primer can attach.
2. Primer annealing - in this step the primer attach to the complementary strands on the single stranded DNA.
3. Extension of primers- DNA polymerase extends the primers and forms double stranded DNA.
The three steps of PCR:
1.Denaturation.
2. Primer annealing
3. Extension of primers.
