Explain any three methods of vectorless gene transfer.

Solution

Vectorless Gene Transfer is when no vector is udes to transfer the desired gene into the host organism. Following are common methods of vectorless gene transfer.

1. Microinjection. Microinjection is the process/technique of introducing foreign genes into a host cell by injecting the DNA directly into the nucleus by using microneedle or micropipette.

2.Use of specific concentraion of divalent cations. specific concentration of divalent cations improves the efficiency of the cells to take up DNA. The divalent cation make the cells competent and thus recombinant DNA can be forced into the cell.

3. Gene Gun or biolistic is the technique of bombarding the cells with microprojectiles (gold or tungsten particles) coated with the foreign DNA with great velocity .

Some More Questions From Biotechnology : Principles and Processes Chapter

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

What are two core techniques that enabled birth of biotechnology ?

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

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Biotechnology : Principles And Processes

Explain any three methods of vectorless gene transfer.

Some More Questions From Biotechnology : Principles and Processes Chapter

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

What are two core techniques that enabled birth of biotechnology ?

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

List three important features necessary for preparing genetically modified organism.

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Entrance Exams Preparation

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For Daily Free Study Material Join wiredfaculty

Biotechnology : Principles And Processes

Explain any three methods of vectorless gene transfer.

Some More Questions From Biotechnology : Principles and Processes Chapter

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

What are two core techniques that enabled birth of biotechnology ?

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.OrWith the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.

List three important features necessary for preparing genetically modified organism.

Mock Test Series

NCERT Sample Papers

Entrance Exams Preparation

What is genetic engineering? Explain briefly the distinct steps common to all genetic engineering technology.
Or
With the help of diagrams show the different steps in the formation of recombinant DNA by action of restriction endonuclease.