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Biotechnology : Principles And Processes

Question
CBSEENBI12003335
Wired Faculty App

Select the correct order of processing of PCR.

  • Extension, primer annealing, denaturation

  • Denaturation, primer annealing, extension

  • Denaturation, extension, primer annealing

  • Primer annealing, denaturation, extension

Solution

B.

Denaturation, primer annealing, extension

Polymerase chain reaction (PCR) is a technique to amplify a selected DNA sequence by a million fold or more. In this procedure, the DNA containing the sequence to be amplified is denatured by heating. This denatured DNA is then annealed to the synthetic oligonucleotide primers. Then Taq polymerase is used to replicate the DNA segment between the sites complementary to olipgonucleotide primer and extend it.

Some More Questions From Biotechnology : Principles and Processes Chapter

What is crown gall tumor ?

Define T-DNA.

Name a technique by which DNA fragments can be separated.

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

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