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Biotechnology : Principles And Processes

Question
CBSEENBI12047106
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Which of the following enzymes is used to join bits of DNA?

  • Ligase

  • Primase

  • Endonuclease

  • DNA polymerase

Solution

A.

Ligase

Ligase is an enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

Primase is an enzyme which is involved in the replication process of DNA. It is a type of RNA Polymerase. It catalyzes the synthesis of a short RNA segment called a primer complementary to a ssDNA template.

Nucleases are enzymes that cut, shorten or degrade nucleic acids. Endonucleases are enzymes which are able to break internal phosphodiester bonds within a DNA molecule.

DNA polymerases are enzymes that synthesize a new strand of DNA complementary to an existing DNA or RNA template.

Some More Questions From Biotechnology : Principles and Processes Chapter

Define T-DNA.

Name a technique by which DNA fragments can be separated.

What are the different tools in Recombinant DNA technology?

Give the full form of PCR. Who developed it ?

What is the source of DNA polymerase i.e., Taq polymerase ?

Define “melting of target DNA”.

What would be the molar concentration of human DNA in a human cell ? Consult your teacher.

Do eukaryotic cells have restriction endonucleases ? Justify your answer.

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size. How did you know ?

Expand EFB.

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