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Biotechnology : Principles And Processes

Question
CBSEENBI12047176
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Which one among the following is just a cloning plasmid not an expression plasmid?

  • pBAD-18-Cam

  • pBCSK

  • pUC18

  • pET

Solution

C.

pUC18

Cloning vector is a small piece of DNA, taken from a virus, a plasmid or the cell of a higher organism that can be stably maintained in an organism. pUC 18 is the most popular E. coli cloning vector.

An expression vector or construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene.

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Expand EFB.

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