During gene cloning, the enzyme used to join the insert DNA with the plasmid vector is

• DNA ligase

• restriction endonuclease

• alkaline phosphatase

• exonuclease

A.

DNA ligase

DNA ligase is a special type of enzyme that is used to seal the doublestranded breaks (i.e., breaks in both complementary strands of DNA) or is used to join insert DNA with the plasmid vector.

Restriction endonuclease are used to make cuts at specific position within DNA.

Exonuclease are used remove nucleotides from the ends of DNA (either 5' or 3') in one strand of duplex.

Alkaline phosphatase are used to responsible for removing phosphate groups from nucleotides, proteins, etc.