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Biotechnology : Principles And Processes

Question
CBSEENBI12002863
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The correct order of steps in Polymerase Chain Reaction (PCR) is

  • Extension, Denaturation, Annealing

  • Annealing, Extension, Denaturation

  • Denaturation, Annealing, Extension

  • Denaturation, Extension, Annealing

Solution

C.

Denaturation, Annealing, Extension

PCR is based on three steps:
(i) Denaturation: denaturation of the template inot single strands.
(ii) Annealing: annealing of primers to each original strand for new strand synthesis.
(iii) Extension:extension of new DNA strands from the primers.

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