Question
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
-
centrifugation
-
polymerase chain reaction
-
electrophoresis
-
restriction mapping
Solution
B.
polymerase chain reaction
C.
electrophoresis
DNA fragments generated by the reaction is simply DNA replication in a test tube. Restriction mapping is the process of obtaining structural information on a piece of DNA by the use of restriction enzymes, e.g., endonucleases that recognise specific 4 to 8 base regions of DNA.