Question
Name the technique used for separation of DNA fragments. What is its principle ? How are they observed ?
Solution
The technique used for separation of DNA fgragments is called gel electrophoresis.
The technique is based on the principle that - when a charged molecule is placed in an electric field they move towards the positive or negative side according to their charge.
When electric field is applied the DNA fragments being negatively charged, move through the gel towards anode and gets seperated.
The DNA fragments are stained with a compound known as ethidium bromide and they are then exposed to UV-radiation. the DNA can be observed as Bright orange coloured bands .
