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Question

List the features required in vectors that  facilitate cloning . With a suitable diagram show the E.coli cloning vector with  restriction sites.

Prabhat Rajore · Accepted Answer

Features required in vectors that facilitate cloning are :
1. Origin of replication (Ori) which helps in autonomous replication and also controls the copy number.
2. Selectable marker which helps in isolation of transformed cell.
3. Cloning sites which provide sites at which foreign DNA can be inserted . These sites also help in selection of recombinants.
4. Vectors like Agrobacterium tumefaciens ( in plants) and retroviruses (in animals) can be made non pathogenic and be used to fascilitate cloning.

Fig. 7.4. E. coli Cloning Vector pBR322 showing restriction sites (Hindlll, EcoRI, BamHI, Sal I, Pvu II, Pst I, Cla l), oriV and antibiotic resistance genes (ampR and tetR). Rop codes for the proteins involved in the replication of the plasmid.

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Biotechnology : Principles And Processes

List the features required in vectors that facilitate cloning . With a suitable diagram show the E.coli cloning vector with restriction sites.

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