Question
Give the overall steps involved in Gene cloning.
Solution
Gene Cloning involves the following steps.
1. Identification and isolation of the desired gene or DNA fragment to be cloned.
1. Identification and isolation of the desired gene or DNA fragment to be cloned.
2. Insertion of the isolated gene in a suitable vector.
3. Introduction of vector into a suitable organism / host cell.
4. Selection of transformed host cells.
5. Multiplication or cloning of the introduced gene in the host organism.
