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Reproduction In Organisms

Question
CBSEENBI12003474
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Which one of the following options is a correct match of phenomenon and its explanation

  • Reverse Transcription PCR - Many copies of a DNA sequence.

  • Central dogma - RNA > DNA > Protein > RNA.

  • RNA silencing - Use of ds-RNA to stop the expression of ss-RNA.

  • Transcription- Process of formation of RNA & proteins.

Solution

A.

Reverse Transcription PCR - Many copies of a DNA sequence.

Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a variant of Polymerase Chain Reaction (PCR), a laboratory technique commonly used in molecular biology to generate many copies of a DNA sequence, a process termed 'amplification'. In RT-PCR, however, an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or cDNA) using the enzyme reverse transcriptase, and the resulting cDNA is amplified using traditional PCR or Real-Time PCR.

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