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Reproduction In Organisms

Question
CBSEENBI12003377
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Which of the following is a cloning vector?

  • DNA of Salmonella typhimurium

  • Ti plasmid

  • Amp' and Tet' loci

  • Ori minus pBR322

Solution

B.

Ti plasmid

The DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called vehicle DNA or cloning vector. The Ti plasmid is present in Agrobacterium tumefaciens. It is a Gram negative soil bacterium that infects a wide range of plants and causes tumorous growth especially at the root or stem junction (crown gall). 

Ti plasmid can be used as a DNA vector by replacing the tumour inducing genes with the gene of interest and a marker gene to enable selection of transformed cells.

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