Strategies for Enhancement in Food Production
Which of the following enhances or induces fusion of protoplasts?
Sodium chloride and potassium chloride
Polyethylene glycol and sodium nitrate
IAA and kinetin
IAA and gibberellins
B.
Polyethylene glycol and sodium nitrate
Polyethylene Glycol (PEG) and sodium nitrate enhances fusion of protoplasts (protoplast fusion). Protoplast fusion is a physical phenomenon which occurs due to the fusion of protoplast (cell without cell wall) of two or more cells. It can be done by mechanically (spontaneous) or inducing agents.
(i) Spontaneous agents Mechanical fusion
(ii) Inducing agents (i) NaNO3 treatment.
(iii) High pH or Ca2+ treatment.
(iv) PEG treatment
(v) Electrofusion.
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