Biotechnology and Its Applications
Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
To obtain a foreign-gene product, following steps should be undertaken:
(i) Identification of DNA with desirable genes.
(ii) Introduction of the identified DNA into suitable host to form recombinant DNA (rDNA).
(iii) Maintenance of introduced DNA in particular host and gene cloning.
(iv) Transfer of the DNA (gene transfer) to its progeny.
(v) Selection of the recombinants from non-recombinants.
(vi) Expression of gene of interest by culturing recombinant cells.
(vii) Culturing of cells in bioreactors for large scale production of desired gene product.
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