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Biotechnology and Its Applications
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Sticky ends are produced by restriction enzymes. These enzymes cut the strand of DNA a little away from the centre of the palindrome sites but between the same two bases on the opposite strands. This leaves single-stranded portions at the ends. These overhanging stretches are called 'sticky ends'.
These are called sticky ends because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.
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