How is transgenic tobacoo plant protected against Meloidegyne incognita ? Explain the process ?

Protection of tobacco plant against Nematode  Meloidegyne incognita by using the method of RNA interference.

1. The specific genes  from the parasite are introduced into the plant using Agrobacterium as the vector.

2. The introduced gene produces both sense/coding RNA and antisense RNA .

3. The two RNA being complementary anneal and become double stranded RNA.

4. This ds RNA initiates RNAi and silences the mRNA of the nematode. 

5. As a result, the parasite cannot live in the transgenic host and the transgenic plant is protected from the pest.