Biotechnology and Its Applications

Question

Can you suggest a method to remove oil (hydrocarbon) from seeds based
on your understanding of rDNA technology and chemistry of oil?

Answer

The genes for the formation of  glycerol or fatty acids  which are the main constituents of oil, should be identified and removed from the seed  by using  enzyme restriction endonucleases. Such DNA molecule should then be treated with DNA ligases to join the broken ends. These cells when grown in a minimum nutrient medium, under aseptic conditions will differentiate into a new variety whose seeds will not have oil in them because the gene producing the oil has been removed.

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