Insect pest resistant Bt cotton plant was developed using

• somaclonal variation

• micropropagation

• somatic hybridisation

• Transgenic technology

D.

Transgenic technology

Bt cotton is a genetically modified variety of cotton producing an insecticide. It was developed by inserting Cry gene coding for Bt toxin into cotton plant genome by using transgenic technology causing cotton to produce this natural insecticide in its tissues. It was obtained from Bacillus thuringiensis. Techniques such as somaclonal variation, somatic hybridisation and micropropagation are the methods that rely as tissue culture techniques of producing plants by using different plant parts. They does not involve the gene level transfer.