Biolistic technique is used in

• tissue culture process

• gene transfer process

• hybridization process

• germplasm conservation process

B.

gene transfer process

Electroporation and biolistics are two physical methods for gene transfection. In biolistics DNA is coated on to the surface of tiny metal particles usually tungeston or gold, which is then shot into the cells.

Electroporation is a molecular biology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs or DNA to be introduced into the cell.