List the steps of preparation of temporary mount of a leaf peel to observe stomata.
The following are the steps of preparation of temporary amount of a leaf peel to observe stomata.
(i) Remove a healthy leaf from the potted plant.
(ii) Remove a part of the peel from the lower surface of the leaf by folding the leaf over and gently pulling the peel apart using forceps.
(iii) Put a few drops of safranin stain in a watch glass.
(iv) After 2-3 minutes take out the peel and place it on a clean glass slide.
(v) Put a drop of glycerin over the peel and place a clean coverslip gently over it.
(vi) Remove the excess stain and glycerin with the help of blotting paper.
(vii) Observe the slide under the low-power and high-power magnifications of the compound microscope.
