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Molecular Basis Of Inheritance

Question
CBSEENBI12002631
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Whose experiments cracked the DNA  and discovered unequivocally that is genetic code is a triplet?

  • Nirenberg and Mathaei

  • Hershey and Chase

  • Morgan and Sturtevant

  • Beadle and Tatum

Solution

A.

Nirenberg and Mathaei

The existence of a triplet code was simply an assumption till 1961 when Nirenberg and Methaei proved its existence by experiment. They were able to synthesise artificial mRNA, which contained only one nitrogenous base, ie, uracil. This synthetic poly-U sequence was then placed in a cell-free system containing protein synthesizing enzymes (extracted from bacterium E. coil) and 20 amino acids together with necessary ATP. During the process, a small polypeptide molecule was produced, which was formed by the linking of phenylalanine. This issuggested that UUU is code for phenylalanine. Nirenberg got Nobel Prize for his contributions.

Some More Questions From Molecular Basis of Inheritance Chapter

List the function of RNA.

List the number of base pairs in :

(i) lambda bacteriophage

(ii) E.coli and

(iii) haploid content of human DNA.

Comment two chains of DNA have antiparallel polarity.

What is the difference between DNAs and DNAase ?

Which was first genetic material ?

Suggest one evidence to prove that RNA was first genetic material.

Why was RNA unstable ?

What made DNA more preferrable as genetic material ?

Process of replication is completed in how much time in E.coli ?

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