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Molecular Basis Of Inheritance

Question
CBSEENBI12002696
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The linking of antibiotic resistance gene with the plasmid vector became possible with

  • DNA ligase

  • endonucleases

  • DNA polymerase

  • exonucleases

Solution

A.

DNA ligase

DNA ligase is used to seal the nicks that remain in recombinant DNA molecule. In fact, DNA ligase joins together the neighbouring nucleotides flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecules is a vector into which the desired DNA fragment has been inserted to enable its cloning in an appropriate host. 
Endonucleases are the enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which has specific base sequences, such endonucleases are known as restriction endonucleases. 
Exonucleases are enzymes that remove one or more nucleotide from the free ends.
DNA polymerase is the enzyme, which causes polymerization of nucleotides during DNA replication.

Some More Questions From Molecular Basis of Inheritance Chapter

Which was first genetic material ?

Suggest one evidence to prove that RNA was first genetic material.

Why was RNA unstable ?

What made DNA more preferrable as genetic material ?

Process of replication is completed in how much time in E.coli ?

What is average rate of polymerization ?

List three components of transcription unit.

Which RNA acts as an adapter ?

Name three kinds of polymerases.

What is splicing ?

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