Sponsor Area

Molecular Basis Of Inheritance

Question
CBSEENBI12002406

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?

  • B – Denaturation at a temperature of about 98°C separatiing the two DNA strands

  • A – Denaturation at a temperature of about 50°C

  • C – Extension in the presence of heat stable DNA polymerase

  • A – Denaturation at a temperature of about 50°C

Solution

C.

C – Extension in the presence of heat stable DNA polymerase

There are three steps in polymerase chain reaction namely.
(i) Denaturation- at 94 degree straight C for DNA strand separation.
(ii) Annealing - binding of primer.
(iii) Extension in presence of DNA polymerase which is heat stable.